Inscripta: The Future of Gene Editing

  • Immense scale: It’s able to run parallel libraries quickly, power diverse edits (insertions, deletions, substitutions, mutations), and accelerate analysis. Compared to other competitors, it is able to use 1.5x less CPU power but run in 2x less time.
  • Greater audience: It’s able to reach a larger community since gene editing expertise is not required for running the application. Due to the technicality of gene editing, it’s inaccessible to many outside a scientific community, but Inscripta’s platform will allow anyone prototyping gene editing to be able to streamline their analysis without much prior knowledge.

Current Products

Onyx Instrument

  • Cell Growth Cuvette: managing/measuring optimal cell growth; Cuvettes are simply containers with straight sides and a square/circular cross-section on the bottom. They are used for holding samples for analysis, and Onyx’s cell growth cuvette is specialized in monitoring cells participating in cell growth.
  • Microfluidic Cell Controller: preparing cells for electroporation; Microfluidics is used to more accurately represent a cell’s environment and control the cell during growth.
  • Microfluidic Cell Transformer: performs electroporation; Electroporation is able to convert animal and plants cells by applying an electric field and introducing genes into the organism.
  • Cell Recovery Cuvette: managing cell growth during recovery; Similar to cell growth cuvettes, they monitor the cell as it “recovers” from the electroporation process.
  • Digital Engineering Processor: normalizing cell editing and growth; This process reviews DNA changes and normalizes them to reduce further growth of the cell outside of the control period.

Onyx Gene Editing Kits

Onyx Genotyping Assays

  • Control Edit Assay: a Sanger sequencing experiment that detects the absence or presence of internal control edits
  • Onyx Barcode Diversity Assay: a library preparation kit for NGS using Illumina — allows for the derivation of distribution of edits
  • Onyx Edit Identification Assay: library preparation kit for WGS using Illumina — can be run with isolates or pooled samples

Onyx Engineering Portal

BioCantor

  • “Genomic feature arithmetic” — allowing for coordinate conversion of various features, binary and unary set operations across arbitrary coordinate systems
  • Abstraction — abstracts the process of converting genomic coordinate systems from the user
  • File format conversion — importing and exported known file formats.

Process

Example of usage of objects for referring to a parent sequence.
  • Location — represents blocked and stranded features represented by zero-based end coordinates; It has three implementations: EmptyLocation, SingleInterval (continuous interval), and CompoundInterval
  • Sequence — holds sequence data
  • Parent — defines parent/child relationships
JSON form of intermediate gene model step.

How Is Inscripta Pushing the Status Quo?

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